Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO4)(2) oscillator

KE. Sheetz, EE. Hoover, R. Carriles, D. Kleinfeld, JA. Squier.
OPTICS EXPRESS 16, 17574 (2008).

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Abstract:
We present a novel Yb:KGd(WO4)(2) oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci. (C) 2008 Optical Society of Americ

Forward references:

Multifocal, Multi-Modal, Photon Counting, Multiphoton Microscopy.
JA. Squier, W. Amir, R. Carriles, E. Chandler, JJ. Field, EE. Hoover, D. Schafer, KE. Sheetz.
2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5 16, 371 (2009).

A diode-pumped continuous-wave Yb:KGW laser with N-g-axis polarized output.
A. Major, D. Sandkuijl, V. Barzda.
LASER PHYSICS LETTERS 6, 779 (2009).

Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy.
R. Carriles, DN. Schafer, KE. Sheetz, JJ. Field, R. Cisek, V. Barzda, AW. Sylvester, JA. Squier.
REVIEW OF SCIENTIFIC INSTRUMENTS 80, 081101 (2009).

Parametric Spatio-Temporal Control of Focusing Laser Pulses.
MA. Coughlan, M. Plewicki, RJ. Levis.
OPTICS EXPRESS 17, 15808 (2009).

High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy.
E. Chandler, E. Hoover, J. Field, K. Sheetz, W. Amir, R. Carriles, SY. Ding, J. Squier.
APPLIED OPTICS 48, 2067 (2009).

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