High-resolution mosaic imaging with multifocal, multiphoton photon-counting microscopy

E. Chandler, E. Hoover, J. Field, K. Sheetz, W. Amir, R. Carriles, SY. Ding, J. Squier.
APPLIED OPTICS 48, 2067 (2009).

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Abstract:
High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multi-photon, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO4 laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters. (C) 2009 Optical Society of Americ

Forward references:

Dispersion-based pulse shaping for multiplexed two-photon fluorescence microscopy.
G. Labroille, RS. Pillai, X. Solinas, C. Boudoux, N. Olivier, E. Beaurepaire, M. Joffre.
OPTICS LETTERS 35, 3444 (2010).

Optical microscopy in photosynthesis.
R. Cisek, L. Spencer, N. Prent, D. Zigmantas, GS. Espie, V. Barzda.
PHOTOSYNTHESIS RESEARCH 102, 111 (2009).

Parametric Spatio-Temporal Control of Focusing Laser Pulses.
MA. Coughlan, M. Plewicki, RJ. Levis.
OPTICS EXPRESS 17, 15808 (2009).

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